Under suggested conditions, BLUelf Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane. This mixture can include all of the proteins associated with a. Proteins were transferred with the iBlot 2 Gel Transfer Device onto nitrocellulose membranes (Cat. A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. I used your Thermo Scientific Unstained Protein MW Marker for a western transfer and got very poor transfer onto the membrane. EA03752BOX) or onto a Novex 420 Tris-glycine gel, WedgeWell format (Cat. Add freshly prepared DTT solution to a final concentration of 50 mM. The BLUelf Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. Method: Western blot analysis of EGFR was performed by loading serially diluted A431 cell lysate onto a NuPAGE 38 Tris-acetate gel (Cat. The 13 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. List Price: Your Price: Log in to see your price. 1.25 ml (5 x 250 µl), 10 pre-stained, Strep -tagged recombinant proteins (10250 kD), including three pink reference bands (25, 50, 75 kD), 250 applications. The BLUelf Prestained Protein Ladder is a combination of 13 pre-stained proteins with molecular weights from 5 to 245 kDa. Precision Plus Protein WesternC Blotting Standards, 1.25 ml. For smeared bands can be different explanations: 1) you load too much. Precision Plus Protein Dual Color Standards, 500 µl 1610374. If not it may indicate there is a problem in one of your buffers. 2.5 μl per well for general Western transferring. Protein Ladders and Standards (Markers) Recombinant Protein Standards (Markers) Prestained Protein Standards. Use 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Custom Recombinant Antibody (rAbs) Services.In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. Annexin V-FITC Apoptosis Detection Kits Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Western blot is often used in research to separate and identify proteins.
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